Techne Prime Pro 48 Real-time qPCR machine

The Prime Pro 48 real time qPCR system is a high specification, economically priced real time thermal cycler that accommodates a unique 48-well polypropylene PCR plate utilising the same geometry as standard 384-well plates, but only 1/8 of the size.This enables users to dramatically reduce the qPCR reagent volumes compared to traditional 96-well instruments, saving users precious sample, whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity. A minimum volume of 5μl is validated, resulting in a more efficient use of expensive and ‘hard to acquire‘ template DNA samples.

KEY FEATURES

High speed

• Ramp rate average 5.5°C/sec. Fast heating and cooling


• 40 cycles in 40 minutes (under 20 minutes optimised) with standard chemistry


• Process up to 40% more samples per hour than a traditional 96-well instrument


• MIQE guidelines compliant software 

Advanced optical technology

• 4 colours for easy multiplexing


• Patented optical system utilised Adaptive LED Control


• Automatically normalises fluorescence. Never saturate the detector. Eliminate cross talk


• Always reads all 48 wells, with all 4 filters at every cycle 

Thermal system

• High resolution melt (HRM) capability


• Patented thermal system. Hollow fluid-filled silver block anodised with gold


• Unparalleled market-leading uniformity ±0.1°C at 95°C


• Up to 5x greater uniformity than any other block-based qPCR instrument 

Reagents

• Techne supply 400 probe based qPCR detection kits


• Kit components are lyophilised for ambient shipping and storage


• Prime Pro 48 also accepts all popular brands of reagents